Hairdressers are integral to community grooming services, but they face occupational health risks due to close contact with clients. Barbershops may act as source of infections such as Hepatitis B, Hepatitis C, HIV, and skin disorders, mainly due to inadequate hygiene practices and a lack of proper infection prevention. Repeated use of razors, towels, and tools without sterilisation further increases this risk. This study aimed to assess the knowledge and practices regarding infection prevention and hygiene among hairdressers in Nadiad city of Gujarat, and develop an informational booklet promoting safe practices. A cross-sectional descriptive study was conducted among 150 hairdressers aged 18-60 years with a minimum of three months of experience, selected using simple random sampling from roadside and shop-based establishments. Data were collected between January and April 2025 using a structured knowledge questionnaire and an observational checklist focusing on hand hygiene, tool sterilisation, and infection awareness. Descriptive (frequency, percentage, mean, SD) and inferential statistics (t-test, chi-square, correlation) were applied. Findings revealed that 59.3 percent of participants had inadequate knowledge, and 54.6 percent demonstrated poor hygiene practices. The mean knowledge score was 9.92 (SD = 4.18), and the mean practice score was 10.65 (SD = 4.83). A significant positive correlation (p < 0.05) was observed between knowledge and practice, indicating that improved awareness enhances hygiene compliance. The study concluded that infection prevention knowledge and practices among hairdressers were suboptimal. An informational booklet was developed to bridge this gap, aiming to reduce infection risks and promote standardised hygiene measures in barbershops.